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FWIW, I looked into this a few months ago. I don't have any cites, but I found some reasonably persuasive articles that light-on-dark is best only for dimly-lit rooms. If you're in a bright office, dark-on-light is best. (And being in a brightly-lit room is probably best for mood and alertness anyway.)

The origins of dark-on-light as "readable" are old-school monochrome computer terminals, where the resolution was so low that if you used light-on-dark, you'd see all the individual pixels - light pixels lit up, dark ones didn't. We're past that now.




Light-on-dark was also for CRTs that bombard your eyes with an electron gun, iirc.

My opinion on this is based on anecdotal evidence only, but my eyes feel less strained with light-on-dark, even to this day, on high res LCDs.

Everyone else, experiment for yourself. Hold all other factors equal, and try one each straight for a few weeks, see if you can feel any difference.




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