One thing others don't seem to have mentioned: While you can do this with any qPCR machine, you would not be allowed to actually use a random qPCR machine for medical testing. You need a special qPCR machine which is installed by the company, then run through a calibration procedure "certify-in-place" which is invalidated if the machine is moved. There is currently a waiting list to buy one of these machines (at least in the U.S.) and they are much more expensive than normal qPCR machines.
