Use the PageSpeed or YSlow Firefox extensions to automatically test your site against some or a majority of these rules or best practices.
Also read http://www.codinghorror.com/blog/archives/000932.html for a perspective on why not all of these rules may apply to your site.
You are right they are not new. Yahoo was one of the first to do rigorous testing to derive these rules and publish them. Along with YSlow, these rules are what kicked off the discussion on those hundreds of other sites. They are also under regular review and testing as we discover new tricks, so check back often.