Nebula most likely ship your samples to China for processing and sequencing because it's so much cheaper. While that might not be a deal breaker for some, I would personally prefer my sample not be shipped elsewhere.
Eh, your results look perfectly fine, especially for a budget sequencing run. Keep in mind there are a lot of repetitive DNA sequences that don't map well (search "mappability track" for more info). Depending on the reference they used, these may even be masked out and thus no alignment can even be attempted.
No one should be BLASTing individual reads... And pretty much no one is going to be assembling with OLC, even for ONT data.
On the products page it says they provide FASTQs, aligned BAM and VCF. Which is exactly what one would expect. They're almost certainly just running the DRAGEN pipeline (or something similar) and giving you the output it creates.
Not for home gamers, no. ONT is fairly low throughput and the prep is surprisingly difficult to do well without experience. You would need to run tens of flow cells to get enough sequencing data.
Not to mention there is a good chunk of required "extra" equipment that is expected (centrifuges, etc) that bring the initial cost to well over $1000. You could buy most thing used, but some of the reagents could be difficult to acquire outside of a traditional lab.
Does it still require a pipettor, or could it work with just a transfer pipet? Their field kit should probably be as minimal as it could be.
Last I looked into it, it was a minimal set of extra equipment -- votexer, centrifuge, etc... nothing unusual for a bio lab. And even most of that could be handled with a good snap of the wrist, if you weren't trying to be super precise.
I have no skin in this particular game (Though I work in the sequencing industry), but...
I don't think anyone is fooled/trying to trick anyone when they say "whole genome sequencing" or that they sequence 100% of the genome. That is the term of art for non-targeted/unenriched sequencing of DNA (nb: it may be RNA/ribosome depleted, so fine it's technically enriched but not in a meaningful way).
Also exons are genic region, are you thinking introns (arguably genic or at least adjacent), or promoters/enhancers and chromatin state?
Lifetime license does not mean lifetime support. I get that that might be annoying, but why or how could they warranty third party software, that they don't control or version themselves, indefinitely?
Maybe. I'll need to get my hands on the license terms which I don't seem to be able to find. They are still offering security support for the product FWIW.
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1. They still get sick, even if asymptomatic, and can bring it home to the house where older people live
2. I don't know if there are published case report / data yet, but anecdotally the new strains are much more likely to cause complications and require hospitalizations for younger children.
3. Even asymptomatic infectious seem to cause "long covid" (Something like a third of long covid cases were asymptomatic). Potentially life-long alterations to lung or heart function doesn't sound like fun
But according to CDC, both people have to be vaccinated to talk to each other inside. This means that if one person is vaccinated you can't see you're immuno-compromised grandparent anyway.
Yes, but if your child is school age -- schools which are now increasingly going to in person instruction -- and you are immunocompromised, what are you going to do? Same question if you live in a multi-generational household, but now there are grandkids going to school and kids going to work.
I see elsewhere you're claiming that long Covid isn't a thing. It is, full stop. Unless you're suggesting that living with lung and heart damage caused by Covid isn't a complication of Covid?
Long COVID is post viral fatigue syndrome. Really sucks when you have it, nothing singularly new about it as far as any available evidence shows. A bunch of people are experiencing it all at the same time, hence the prominence in the media. "Long flu" is much more diffuse in time, so not media-worthy. Mine was 3 months of hell at 18 years old.
The answer to "Why are they investigating?" is simple and, in fact, driven in part by the pharmaceutical industry itself.
First: The rate of thrombosis in AZ recipients is the less than it is in the general population -- Gen pop, ~.1% [1], AZ ~ 0.001% [2]. There is no evidence that the vaccine causes substantially higher risk. Also, the populations being prioritized for vaccination are a higher risk population for DVT to begin with.[1]
AZ, along with effectively every pharma company out there of note, takes the reporting of adverse drug responses (ADRs) very, very seriously. Both FDA and EMA require ALL companies that produce a labeled product (aka a drug you can "buy" and isn't only available in a trial setting) to investigate and report on every instance of a reported potential ADR. Companies want to investigate because they want to be able to keep selling their drugs. Regulators want to investigate because they want to limit ADRs as much as possible.
From the therapeutic point of view, it is bad if the treatment causes ADRs but also some may be unavoidable because of how the treatment works -- think chemo and cancer. The safety window for a drug is determined by balancing the therapeutic gain of treatment (usually, shorter time to recovery, increased QOL, or, in the case of cancers, increased life span/PFS) with the number and severity of known adverse effects. You might hear about cancer patients "cycling" their treatments, this is to allow time for the body to recover from known/expected ADRs.
Any way, this was a long winded way of saying every entity involved -- drug manufacturer, regulators, doctors, patients -- wants reports of ADRs investigated.
> AZ, along with effectively every pharma company out there of note, takes the reporting of adverse drug responses (ADRs) very, very seriously.
This is the reason that a lot of medications list the thing they're treating as a side effect. It doesn't work perfectly, so people report they're still having the condition, and that has to go on the list.
